CRISPResso version 2.3.4
[Command used]:
/var/www/webapps/CRISPRessoWEB/.pixi/envs/default/bin/CRISPResso -r1 allele_specific.fastq.gz -a CGAGAGCCGCAGCCATGAACGGCACAGAGGGCCCCAATTTTTATGTGCCCTTCTCCAACGTCACAGGCGTGGTGCGGAGCCACTTCGAGCAGCCGCAGTACTACCTGGCGGAACCATGGCAGTTCTCCATGCTGGCAGCGTACATGTTCCTGCTCATCGTGCTGGG,CGAGAGCCGCAGCCATGAACGGCACAGAGGGCCCCAATTTTTATGTGCCCTTCTCCAACGTCACAGGCGTGGTGCGGAGCCCCTTCGAGCAGCCGCAGTACTACCTGGCGGAACCATGGCAGTTCTCCATGCTGGCAGCGTACATGTTCCTGCTCATCGTGCTGGG -an P23H,WT -g GTGCGGAGCCACTTCGAGCAGC --write_cleaned_report --place_report_in_output_folder --use_matplotlib

[Execution log]:
CRISPRessoPro v1.0.1 installed
Computing quantification windows
Added 0 guides with flexible matching
	Original flexiguides: ['None']
	Found guides: []
	Mismatch locations: []
Added 0 guides with flexible matching
	Original flexiguides: ['None']
	Found guides: []
	Mismatch locations: []
Counting reads in input
Counting reads after preprocessing...
Aligning sequences...
Finished reading fastq file; 4387 unique reads found of 25000 total reads found 
Processing Reads; 0 Completed out of 4387 Unique Reads
Finished reads; N_TOT_READS: 25000 N_COMPUTED_ALN: 4219 N_CACHED_ALN: 20407 N_COMPUTED_NOTALN: 168 N_CACHED_NOTALN: 206
Done!
Quantifying indels/substitutions...
Done!
Calculating allele frequencies...
Done!
Saving processed data...
Making Plots...
Done!
Removing Intermediate files...
Disproportionate percentages of reads were aligned to amplicon: P23H, Percent of aligned reads aligned to this amplicon: 45.21%.
Disproportionate percentages of reads were aligned to amplicon: WT, Percent of aligned reads aligned to this amplicon: 51.0%.
>=1.0% of reads have modifications at the start or end. Total reads: 24626, Irregular reads: 2114.
>=0.2% of substitutions were outside of the quantification window. Total substitutions: 7154, Substitutions outside window: 6891.
Pro report written to CRISPResso_on_allele_specific/CRISPResso2_report.html
                                                                               
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Analysis Complete!