CRISPResso version 2.3.4
[Command used]:
/var/www/webapps/CRISPRessoWEB/.pixi/envs/default/bin/CRISPResso --fastq_r1 base_editor.fastq.gz --amplicon_seq GGCCCCAGTGGCTGCTCTGGGGGCCTCCTGAGTTTCTCATCTGTGCCCCTCCCTCCCTGGCCCAGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATCGATGTCACCTCCAATGACTAGGGTGG --guide_seq GAGTCCGAGCAGAAGAAGAA --quantification_window_size 20 --quantification_window_center -10 --base_editor_output --write_cleaned_report --place_report_in_output_folder --use_matplotlib

[Execution log]:
CRISPRessoPro v1.0.1 installed
Computing quantification windows
Added 0 guides with flexible matching
	Original flexiguides: ['None']
	Found guides: []
	Mismatch locations: []
Counting reads in input
Counting reads after preprocessing...
Aligning sequences...
Finished reading fastq file; 3939 unique reads found of 25000 total reads found 
Processing Reads; 0 Completed out of 3939 Unique Reads
Finished reads; N_TOT_READS: 25000 N_COMPUTED_ALN: 3909 N_CACHED_ALN: 21061 N_COMPUTED_NOTALN: 30 N_CACHED_NOTALN: 0
Done!
Quantifying indels/substitutions...
Done!
Calculating allele frequencies...
Done!
Saving processed data...
Making Plots...
Done!
Removing Intermediate files...
>=1.0% of reads have modifications at the start or end. Total reads: 24970, Irregular reads: 24970.
>=0.2% of substitutions were outside of the quantification window. Total substitutions: 17498, Substitutions outside window: 4061.
Pro report written to CRISPResso_on_base_editor/CRISPResso2_report.html
                                                                               
                                        _                                      
                                       '  )                                    
                                       .-'                                     
                                      (____                                    
                                   C)|     \                                   
                                     \     /                                   
                                      \___/                                    

Analysis Complete!