CRISPResso version 2.3.4
[Command used]:
/var/www/webapps/CRISPRessoWEB/.pixi/envs/default/bin/CRISPResso -r1 hdr.fastq.gz -a acatttgcttctgacacaactgtgttcactagcaacctcaaacagacaccatggtgcatctgactcctgTggagaagtctgccgttactgccctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcaggttggtatcaaggtta -e acatttgcttctgacacaactgtgttcactagcaacctcaaacagacaccatggtgcaCctgactccGgaggagaagtctgccgttactgcGctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcaggttggtatcaaggtta -c atggtgcatctgactcctgTggagaagtctgccgttactgccctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcag -g TGCACCATGGTGTCTGTTTG --write_cleaned_report --place_report_in_output_folder --use_matplotlib

[Execution log]:
CRISPRessoPro v1.0.1 installed
Computing quantification windows
Added 0 guides with flexible matching
	Original flexiguides: ['None']
	Found guides: []
	Mismatch locations: []
Added 0 guides with flexible matching
	Original flexiguides: ['None']
	Found guides: []
	Mismatch locations: []
Counting reads in input
Counting reads after preprocessing...
Aligning sequences...
Finished reading fastq file; 1107 unique reads found of 25000 total reads found 
Processing Reads; 0 Completed out of 1107 Unique Reads
Finished reads; N_TOT_READS: 25000 N_COMPUTED_ALN: 1107 N_CACHED_ALN: 23893 N_COMPUTED_NOTALN: 0 N_CACHED_NOTALN: 0
Done!
Quantifying indels/substitutions...
Done!
Calculating allele frequencies...
Done!
Saving processed data...
Making Plots...
Done!
Removing Intermediate files...
Disproportionate percentages of reads were aligned to amplicon: Reference, Percent of aligned reads aligned to this amplicon: 93.66%.
Disproportionate percentages of reads were aligned to amplicon: HDR, Percent of aligned reads aligned to this amplicon: 6.31%.
>=0.2% of substitutions were outside of the quantification window. Total substitutions: 3649, Substitutions outside window: 3500.
Pro report written to CRISPResso_on_hdr/CRISPResso2_report.html
                                                                               
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Analysis Complete!