Not sure where to start?
Manual
Source on Github
Examples
  • NHEJ (Non-homologous end joining)
  • Multiple alleles
  • Base-editors
  • HDR (Homology directed repair)
  • Prime editing
  • Batch mode

CRISPResso2

Analysis of genome editing outcomes from deep sequencing data

Not sure where to start?
Manual
Source on Github
Examples
  • NHEJ (Non-homologous end joining)
  • Multiple alleles
  • Base-editors
  • HDR (Homology directed repair)
  • Prime editing
  • Batch mode

Experimental design



Sample name
Amplicon
sgRNA

Sample Name
Amplicon Name/s
Minimum homology
Base editing

Prime editing
pegRNA spacer sequence
pegRNA extension sequence
pegRNA extension quantification window size
pegRNA scaffold minimum match length
Nicking sgRNA
Scaffold sequence
Override prime edited reference sequence

Quantification window
Quantification window center
Quantification window size
Plot window size

HDR

Exon specification

Quality filtering and trimming
Minimum average read quality greater than
Minimum single bp quality greater than
Replace bases with N that have a quality lower than
Exclude bp from the left side
Exclude bp from the right side

By clicking the submit button, I agree to use CRISPResso2 in accordance with Partners' Terms and Conditions.

For analysis of amplicon pools, WGS data, and comparing multiple conditions, command line utilities for running CRISPResso2 on your machine or cluster are available at: https://github.com/pinellolab/CRISPResso2.